Identifying a Carotenoid Cleavage Dioxygenase 4a Gene and Its Efficient Agrobacterium-Mediated Genetic Transformation in Bixa orellana L.
Identifieur interne : 000293 ( Main/Exploration ); précédent : 000292; suivant : 000294Identifying a Carotenoid Cleavage Dioxygenase 4a Gene and Its Efficient Agrobacterium-Mediated Genetic Transformation in Bixa orellana L.
Auteurs : Mohan Sankari [Inde] ; Hridya Hemachandran [Inde] ; Amirtha Anantharaman [Inde] ; Subramanian Babu [Inde] ; Renata Rivera Madrid [Mexique] ; George Priya Doss C [Inde] ; Devanand P. Fulzele [Inde] ; Ramamoorthy Siva [Inde]Source :
- Applied biochemistry and biotechnology [ 1559-0291 ] ; 2016.
Descripteurs français
- KwdFr :
- Agrobacterium (génétique), Arabidopsis (enzymologie), Arabidopsis (génétique), Bixaceae (enzymologie), Bixaceae (génétique), Caroténoïdes (génétique), Caroténoïdes (métabolisme), Crocus (enzymologie), Crocus (génétique), Dioxygenases (génétique), Dioxygenases (métabolisme), Oxygénases (génétique), Oxygénases (métabolisme), Phylogenèse (MeSH), Protéines d'Arabidopsis (génétique), Protéines d'Arabidopsis (métabolisme), Transformation génétique (MeSH).
- MESH :
- enzymologie : Arabidopsis, Bixaceae, Crocus.
- génétique : Agrobacterium, Arabidopsis, Bixaceae, Caroténoïdes, Crocus, Dioxygenases, Oxygénases, Protéines d'Arabidopsis.
- métabolisme : Caroténoïdes, Dioxygenases, Oxygénases, Protéines d'Arabidopsis.
- Phylogenèse, Transformation génétique.
English descriptors
- KwdEn :
- Agrobacterium (genetics), Arabidopsis (enzymology), Arabidopsis (genetics), Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), Bixaceae (enzymology), Bixaceae (genetics), Carotenoids (genetics), Carotenoids (metabolism), Crocus (enzymology), Crocus (genetics), Dioxygenases (genetics), Dioxygenases (metabolism), Oxygenases (genetics), Oxygenases (metabolism), Phylogeny (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Arabidopsis Proteins, Carotenoids, Dioxygenases, Oxygenases.
- enzymology : Arabidopsis, Bixaceae, Crocus.
- genetics : Agrobacterium, Arabidopsis, Bixaceae, Crocus.
- chemical , metabolism : Arabidopsis Proteins, Carotenoids, Dioxygenases, Oxygenases.
- Phylogeny, Transformation, Genetic.
Abstract
Carotenoids are metabolized to apocarotenoids through the pathway catalysed by carotenoid cleavage oxygenases (CCOs). The apocarotenoids are economically important as it is known to have therapeutic as well as industrial applications. For instance, bixin from Bixa orellana and crocin from Crocus sativus are commercially used as a food colourant and cosmetics since prehistoric time. In our present study, CCD4a gene has been identified and isolated from leaves of B. orellana for the first time and named as BoCCD4a; phylogenetic analysis was carried out using CLUSTAL W. From sequence analysis, BoCCD4a contains two exons and one intron, which was compared with the selected AtCCD4, RdCCD4, GmCCD4 and CmCCD4a gene. Further, the BoCCD4a gene was cloned into pCAMBIA 1301, transformed into Agrobacterium tumefaciens EHA105 strain and subsequently transferred into hypocotyledons and callus of B. orellana by agro-infection. Selection of stable transformation was screened on the basis of PCR detection by using GUS and hptII specific primer, which was followed by histochemical characterization. The percent transient GUS expression in hypocotyledons and callus was 84.4 and 80 %, respectively. The expression of BoCCD4a gene in B. orellana was confirmed through RT-PCR analysis. From our results, the sequence analysis of BoCCD4a gene of B. orellana was closely related to the CsCCD4 gene of C. sativus, which suggests this gene may have a role in various processes such as fragrance, insect attractant and pollination.
DOI: 10.1007/s12010-016-2025-8
PubMed: 26922728
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Calle 43 No. 130, Col. Chuburnade Hidalgo, 97200, Merida, Yucatan, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Cenro de Investigacion Cientifica de Yucatan, A.C. Calle 43 No. 130, Col. Chuburnade Hidalgo, 97200, Merida, Yucatan</wicri:regionArea><wicri:noRegion>Yucatan</wicri:noRegion></affiliation></author><author><name sortKey="C, George Priya Doss" sort="C, George Priya Doss" uniqKey="C G" first="George Priya Doss" last="C">George Priya Doss C</name><affiliation wicri:level="1"><nlm:affiliation>School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu</wicri:regionArea><wicri:noRegion>Tamil Nadu</wicri:noRegion></affiliation></author><author><name sortKey="Fulzele, Devanand P" sort="Fulzele, Devanand P" uniqKey="Fulzele D" first="Devanand P" last="Fulzele">Devanand P. Fulzele</name><affiliation wicri:level="1"><nlm:affiliation>Plant Biotechnology and Secondary Metabolites Section, NA&BTD, Bhabha Atomic Research Centre, Mumbai, Maharashtra, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Plant Biotechnology and Secondary Metabolites Section, NA&BTD, Bhabha Atomic Research Centre, Mumbai, Maharashtra</wicri:regionArea><wicri:noRegion>Maharashtra</wicri:noRegion></affiliation></author><author><name sortKey="Siva, Ramamoorthy" sort="Siva, Ramamoorthy" uniqKey="Siva R" first="Ramamoorthy" last="Siva">Ramamoorthy Siva</name><affiliation wicri:level="1"><nlm:affiliation>School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India. rsiva77in@rediffmail.com.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu</wicri:regionArea><wicri:noRegion>Tamil Nadu</wicri:noRegion></affiliation></author></analytic><series><title level="j">Applied biochemistry and biotechnology</title><idno type="eISSN">1559-0291</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium (genetics)</term><term>Arabidopsis (enzymology)</term><term>Arabidopsis (genetics)</term><term>Arabidopsis Proteins (genetics)</term><term>Arabidopsis Proteins (metabolism)</term><term>Bixaceae (enzymology)</term><term>Bixaceae (genetics)</term><term>Carotenoids (genetics)</term><term>Carotenoids (metabolism)</term><term>Crocus (enzymology)</term><term>Crocus (genetics)</term><term>Dioxygenases (genetics)</term><term>Dioxygenases (metabolism)</term><term>Oxygenases (genetics)</term><term>Oxygenases (metabolism)</term><term>Phylogeny (MeSH)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium (génétique)</term><term>Arabidopsis (enzymologie)</term><term>Arabidopsis (génétique)</term><term>Bixaceae (enzymologie)</term><term>Bixaceae (génétique)</term><term>Caroténoïdes (génétique)</term><term>Caroténoïdes (métabolisme)</term><term>Crocus (enzymologie)</term><term>Crocus (génétique)</term><term>Dioxygenases (génétique)</term><term>Dioxygenases (métabolisme)</term><term>Oxygénases (génétique)</term><term>Oxygénases (métabolisme)</term><term>Phylogenèse (MeSH)</term><term>Protéines d'Arabidopsis (génétique)</term><term>Protéines d'Arabidopsis (métabolisme)</term><term>Transformation génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Arabidopsis Proteins</term><term>Carotenoids</term><term>Dioxygenases</term><term>Oxygenases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Arabidopsis</term><term>Bixaceae</term><term>Crocus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Arabidopsis</term><term>Bixaceae</term><term>Crocus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium</term><term>Arabidopsis</term><term>Bixaceae</term><term>Crocus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium</term><term>Arabidopsis</term><term>Bixaceae</term><term>Caroténoïdes</term><term>Crocus</term><term>Dioxygenases</term><term>Oxygénases</term><term>Protéines d'Arabidopsis</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Arabidopsis Proteins</term><term>Carotenoids</term><term>Dioxygenases</term><term>Oxygenases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Caroténoïdes</term><term>Dioxygenases</term><term>Oxygénases</term><term>Protéines d'Arabidopsis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Phylogeny</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Phylogenèse</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Carotenoids are metabolized to apocarotenoids through the pathway catalysed by carotenoid cleavage oxygenases (CCOs). The apocarotenoids are economically important as it is known to have therapeutic as well as industrial applications. For instance, bixin from Bixa orellana and crocin from Crocus sativus are commercially used as a food colourant and cosmetics since prehistoric time. In our present study, CCD4a gene has been identified and isolated from leaves of B. orellana for the first time and named as BoCCD4a; phylogenetic analysis was carried out using CLUSTAL W. From sequence analysis, BoCCD4a contains two exons and one intron, which was compared with the selected AtCCD4, RdCCD4, GmCCD4 and CmCCD4a gene. Further, the BoCCD4a gene was cloned into pCAMBIA 1301, transformed into Agrobacterium tumefaciens EHA105 strain and subsequently transferred into hypocotyledons and callus of B. orellana by agro-infection. Selection of stable transformation was screened on the basis of PCR detection by using GUS and hptII specific primer, which was followed by histochemical characterization. The percent transient GUS expression in hypocotyledons and callus was 84.4 and 80 %, respectively. The expression of BoCCD4a gene in B. orellana was confirmed through RT-PCR analysis. From our results, the sequence analysis of BoCCD4a gene of B. orellana was closely related to the CsCCD4 gene of C. sativus, which suggests this gene may have a role in various processes such as fragrance, insect attractant and pollination. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26922728</PMID><DateCompleted><Year>2017</Year><Month>01</Month><Day>13</Day></DateCompleted><DateRevised><Year>2017</Year><Month>01</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1559-0291</ISSN><JournalIssue CitedMedium="Internet"><Volume>179</Volume><Issue>5</Issue><PubDate><Year>2016</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Applied biochemistry and biotechnology</Title><ISOAbbreviation>Appl Biochem Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Identifying a Carotenoid Cleavage Dioxygenase 4a Gene and Its Efficient Agrobacterium-Mediated Genetic Transformation in Bixa orellana L.</ArticleTitle><Pagination><MedlinePgn>697-714</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12010-016-2025-8</ELocationID><Abstract><AbstractText>Carotenoids are metabolized to apocarotenoids through the pathway catalysed by carotenoid cleavage oxygenases (CCOs). The apocarotenoids are economically important as it is known to have therapeutic as well as industrial applications. For instance, bixin from Bixa orellana and crocin from Crocus sativus are commercially used as a food colourant and cosmetics since prehistoric time. In our present study, CCD4a gene has been identified and isolated from leaves of B. orellana for the first time and named as BoCCD4a; phylogenetic analysis was carried out using CLUSTAL W. From sequence analysis, BoCCD4a contains two exons and one intron, which was compared with the selected AtCCD4, RdCCD4, GmCCD4 and CmCCD4a gene. Further, the BoCCD4a gene was cloned into pCAMBIA 1301, transformed into Agrobacterium tumefaciens EHA105 strain and subsequently transferred into hypocotyledons and callus of B. orellana by agro-infection. Selection of stable transformation was screened on the basis of PCR detection by using GUS and hptII specific primer, which was followed by histochemical characterization. The percent transient GUS expression in hypocotyledons and callus was 84.4 and 80 %, respectively. The expression of BoCCD4a gene in B. orellana was confirmed through RT-PCR analysis. From our results, the sequence analysis of BoCCD4a gene of B. orellana was closely related to the CsCCD4 gene of C. sativus, which suggests this gene may have a role in various processes such as fragrance, insect attractant and pollination. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sankari</LastName><ForeName>Mohan</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hemachandran</LastName><ForeName>Hridya</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Anantharaman</LastName><ForeName>Amirtha</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Babu</LastName><ForeName>Subramanian</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Madrid</LastName><ForeName>Renata Rivera</ForeName><Initials>RR</Initials><AffiliationInfo><Affiliation>Cenro de Investigacion Cientifica de Yucatan, A.C. Calle 43 No. 130, Col. Chuburnade Hidalgo, 97200, Merida, Yucatan, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>C</LastName><ForeName>George Priya Doss</ForeName><Initials>GP</Initials><AffiliationInfo><Affiliation>School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fulzele</LastName><ForeName>Devanand P</ForeName><Initials>DP</Initials><AffiliationInfo><Affiliation>Plant Biotechnology and Secondary Metabolites Section, NA&BTD, Bhabha Atomic Research Centre, Mumbai, Maharashtra, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Siva</LastName><ForeName>Ramamoorthy</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India. rsiva77in@rediffmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>02</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Biochem Biotechnol</MedlineTA><NlmUniqueID>8208561</NlmUniqueID><ISSNLinking>0273-2289</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029681">Arabidopsis Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>36-88-4</RegistryNumber><NameOfSubstance UI="D002338">Carotenoids</NameOfSubstance></Chemical><Chemical><RegistryNumber>9L7T4VB66G</RegistryNumber><NameOfSubstance UI="C004588">bixin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="C000592331">CCD4 protein, Arabidopsis</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.13.-</RegistryNumber><NameOfSubstance UI="D010105">Oxygenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.13.-</RegistryNumber><NameOfSubstance UI="C500451">carotenoid oxygenase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.13.11.-</RegistryNumber><NameOfSubstance UI="D049308">Dioxygenases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D060054" MajorTopicYN="N">Agrobacterium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029681" MajorTopicYN="N">Arabidopsis Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031212" MajorTopicYN="N">Bixaceae</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002338" MajorTopicYN="N">Carotenoids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027622" MajorTopicYN="N">Crocus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049308" MajorTopicYN="N">Dioxygenases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010105" MajorTopicYN="N">Oxygenases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="Y">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agro-infection</Keyword><Keyword MajorTopicYN="N">Apocarotenoids</Keyword><Keyword MajorTopicYN="N">Bixa orellana</Keyword><Keyword MajorTopicYN="N">CCD4a</Keyword><Keyword MajorTopicYN="N">Carotenoid cleavage dioxygenase</Keyword><Keyword MajorTopicYN="N">RT-PCR</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>10</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>02</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>2</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>2</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>1</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26922728</ArticleId><ArticleId IdType="doi">10.1007/s12010-016-2025-8</ArticleId><ArticleId IdType="pii">10.1007/s12010-016-2025-8</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li><li>Mexique</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Sankari, Mohan" sort="Sankari, Mohan" uniqKey="Sankari M" first="Mohan" last="Sankari">Mohan Sankari</name></noRegion><name sortKey="Anantharaman, Amirtha" sort="Anantharaman, Amirtha" uniqKey="Anantharaman A" first="Amirtha" last="Anantharaman">Amirtha Anantharaman</name><name sortKey="Babu, Subramanian" sort="Babu, Subramanian" uniqKey="Babu S" first="Subramanian" last="Babu">Subramanian Babu</name><name sortKey="C, George Priya Doss" sort="C, George Priya Doss" uniqKey="C G" first="George Priya Doss" last="C">George Priya Doss C</name><name sortKey="Fulzele, Devanand P" sort="Fulzele, Devanand P" uniqKey="Fulzele D" first="Devanand P" last="Fulzele">Devanand P. Fulzele</name><name sortKey="Hemachandran, Hridya" sort="Hemachandran, Hridya" uniqKey="Hemachandran H" first="Hridya" last="Hemachandran">Hridya Hemachandran</name><name sortKey="Siva, Ramamoorthy" sort="Siva, Ramamoorthy" uniqKey="Siva R" first="Ramamoorthy" last="Siva">Ramamoorthy Siva</name></country><country name="Mexique"><noRegion><name sortKey="Madrid, Renata Rivera" sort="Madrid, Renata Rivera" uniqKey="Madrid R" first="Renata Rivera" last="Madrid">Renata Rivera Madrid</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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{{Explor lien
|wiki= Bois
|area= AgrobacTransV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26922728
|texte= Identifying a Carotenoid Cleavage Dioxygenase 4a Gene and Its Efficient Agrobacterium-Mediated Genetic Transformation in Bixa orellana L.
}}
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| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
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